The CQ1 enables 3D imaging and quantification of live cell clusters, such as spheroids within a 3D culture vessel, as they are, keeping the cells intact. The system has the Microlensed Dual Spinning Disk with Laser Illumination, 4 lasers (405nm, 488 nm, 561nm, and 640nm) and 5 Objectives (4x, 10x Apo, 20x ELWD, 20x Apo, and 40x Apo). It also has a motorized stage which is heated and capable of incubation for long time-lapse imaging and it can scan multi-well in addition to slides and 35mm petri dishes.